Lyticase from Arthrobacter Luteus
SIGMA/SRE0018 - Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein
Synonym: Lyticase from Arthrobacter luteus
CAS Number: 37340-57-1
MDL Number: MFCD00131560
Product Type: Chemical
Lyticase specificity: Yeast β-glucan is a polymer of β-(1-3)-D-glycopyranosyl units with branching at β-(1-6)-D-glycopyranosyl units.
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial (Arthrobacter luteus) |
| color | light brown |
| composition | Protein, ≥20% biuret |
| concentration | 20 % protein Biuret |
| form | lyophilized powder |
| Quality Level | 400  |
| shipped in | wet ice |
| specific activity | ≥2000 units/mg protein |
| storage temp. | −20°C |
| Application: | For isolation of nucleic acids, lyticase is used in the lysis of yeast cell walls (e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species). |
| Biochem/physiol Actions: | Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan. |
| General description: | Lyticase is an enzyme obtained from Arthrobacter Luteus that can extract DNA from yeast by inducing partial spheroplast formation which are later lysed to release DNA. It is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities. |
| Other Notes: | One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2000 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |