Butyrylcholinesterase from equine serum
SIGMA/SRE0020 - Suitable for manufacturing of diagnostic kits and reagents, ≥900 units/mg protein
Synonym: Butyrylcholinesterase from equine serum; Acylcholine acyl-hydrolase; Choline esterase, butyryl; Pseudocholinesterase
CAS Number: 9001-08-5
EC Number: 232-579-2
MDL Number: MFCD00081455
Product Type: Chemical
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the simple uncoupled assay using acetylcholine which can be measured titrimetrically.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the coupled assay using choline oxidase and peroxidase.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using DTNB detection.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using ferricyanate detection.
Butyrylcholinesterase (Cat. No. SRE0020) was dissolved in native sample buffer, PAGE was run on a 4–16% Bis-Tris gel
| application(s) | diagnostic assay manufacturing |
| assay | (colorimetric) |
| color | beige |
| form | lyophilized powder |
| impurities | ≤12% |
| Quality Level | 400  |
| shipped in | wet ice |
| solubility | soluble (Native Sample buffer) |
| specific activity | ≥900 units/mg protein |
| storage temp. | −20°C |
| Other Notes: | One unit will hydrolyze 1.0 micromole of butyrylcholine to choline and butyrate per minute at pH 8.0 at 37 deg C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | (colorimetric) |
| activity | specific activity: ≥900 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.8 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |