Granzyme B human
SIGMA/SRE0021 - recombinant, expressed in insect cells, ≥90% (SDS-PAGE)
Synonym: Granzyme B human; Cytotoxic T-lymphocyte proteinase 2; Granzyme-2; GranzymeB; Lymphocyte protease; SECT; T-cell serine protease 1-3E
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| packaging | pkg of 5 μg |
| potency | >0.004 units/mg |
| Quality Level | 200  |
| recombinant | expressed in insect cells |
| shipped in | wet ice |
| specific activity | ≥40 units/μg |
| storage temp. | −70°C |
| UniProt accession no. | P10144  |
| Biochem/physiol Actions: | Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell. |
| General description: | Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B. |
| Other Notes: | One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl2. |
| Physical form: | Lyophilized powder, containing HEPES buffer (original solution at pH 7.5) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥40 units/μg |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |