β-Glucuronidase from abalone
SIGMA/SRE0023 - Purified, aqueous solution, β-glucuronidase 150,000-250,000 units/mL, β-glucuronidase ≥20,000,000 units/g protein
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
LC-MS analysis of codeine-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. The limpet-derived enzyme G8132 performed best with this substrate, with reaction completion within 3–4 hours at 60 °C at 1 unit per mL. Comparative data above was performed at 60 °C at 10 unit per mL.
LC-MS analysis of hydromorphone-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. Molluscan enzymes performed similarly. The reaction was complete within 3–4 hours at 60 °C at 1 unit per mL. The E. coli enzymes exhibited low specificity for this substrate.
Enzymatic hydrolysis rates of six glucuronide substrates at 1,000 ng/mL in synthetic urine using heavy-labeled internal standards at 250 ng/mL. Hydrolysis was performed at 1.0 units/mL α-glucuronidase in acetate buffer at pH 5.0.
Enzymatic hydrolysis rates of six glucuronide substrates at 1,000 ng/mL in synthetic urine using heavy-labeled internal standards at 250 ng/mL. Hydrolysis was performed at 10 units/mL α-glucuronidase in acetate buffer at pH 5.0.
| β-glucuronidase activity | ≥20,000,000 units/g protein |
| 150,000-250,000 units/mL | |
| form | aqueous solution |
| Quality Level | 200  |
| secondary activity | ≤7,500 units/mL sulfatase |
| storage temp. | 2-8°C |
| Application: | Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine. |
| Biochem/physiol Actions: | β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain. |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay). |
| Packaging: | 25, 50 mL in glass bottle |
| Physical form: | Supplied as an aqueous solution containing |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | β-glucuronidase activity: ≥20,000,000 units/g protein; β-glucuronidase activity: 150,000-250,000 units/mL; secondary activity: ≤7,500 units/mL sulfatase |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.2.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |