Glyceraldehyde-3-phosphate dehydrogenase human

SIGMA/SRE0024 - recombinant, expressed in Escherichia coli

Synonym: D-Glyceraldehyde-3-phosphate: NAD⁺ oxidoreductase (phosphorylating); GAPDH; Triosephosphate dehydrogenase

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-SRE0024-100UG	100 µg	$539.00 1/EA		Add To Favorites

SDS-PAGE Glyceraldehyde-3-phosphate dehydrogenase human (Cat. No. SRE0024) was assessed on SDS-PAGE.

Properties
Descriptions
Safety Info.
Basic Data

Quality Level	200
recombinant	expressed in E. coli
specific activity	≥80 units/mg protein
storage temp.	−20°C

Application:	Human glyceraldehyde-3-phosphate dehydrogenase has been used as a positive control for hydrogen sulfide treatment to detect sulfhydration of protein targets.
Biochem/physiol Actions:	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy) and huntingtin. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. GAPDH is also a component of the functional GAIT (interferon-?-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.
General description:	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) localizes to the cytoplasm but can be translocated to the nucleus depending on cellular conditions. It is a tetramer containing identical chains. The gene encoding GAPDH is localized on human chromosome 12p13.
Other Notes:	Alternative number: CAS Number 9001-50-7
Other Notes:	One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per minute in a coupled system with 3-phosphoglyceric phosphokinase (3-PGK) at pH 7.6 at 25 °C
Physical form:	This product is recombinant, human GAPDH expressed in E. coli with an N-terminal histidine tag and has a predicted molecular mass of 37,984 Da. The product is lyophilized from a buffered solution with stabilizers.

Symbol	GHS07
Signal word	Warning
Hazard statements	H319
Precautionary statements	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥80 units/mg protein
Storage Temp.	−20°C
Enzyme Commission (EC) Number	1.2.1.12
UNSPSC	12352204

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