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Phosphatase substrate

SIGMA/SRE0026 - Suitable for manufacturing of diagnostic kits and reagents

Synonym: Phosphatase substrate; 4-Nitrophenyl phosphate disodium salt hexahydrate; pNPP disodium salt hexahydrate

CAS Number: 333338-18-4
Empirical Formula (Hill Notation): C6H4NNa2O6P · 6H2O
Molecular Weight: 371.14
EC Number: 224-246-5
MDL Number: MFCD00066288
Linear Formula: O2NC6H4OP(O)(ONa)2 · 6H2O
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SRE0026-1G 1 g
 $172.00
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45-SRE0026-5G 5 g
 $493.00
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45-SRE0026-10G 10 g
 $844.00
1/EA		 Add To Favorites
45-SRE0026-25G 25 g
 $1890.00
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45-SRE0026-50G 50 g
 $3390.00
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pNPP substrates develop the yellow end-product, p-nitrophenol, when hydrolyzed by alkaline phosphatase. Since this system forms a soluble end product, it is not recommended for blotting or histochemistry.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: SRE0026-50G.

 

application(s) diagnostic assay manufacturing
assay ≥97% (HPLC)
form powder
InChI 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key KAKKHKRHCKCAGH-UHFFFAOYSA-L
Quality Level 400 
SMILES string O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
solubility water: 100 mg/mL, clear to slightly hazy
storage temp. −20°C
Application: Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Application: Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
Other Notes: Substrate for the determination of acid and alkaline phosphatases.
Packaging: 5, 10, 25 g in glass bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥97% (HPLC)
Storage Temp. −20°C
UNSPSC 12352204

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