β-Glucuronidase from abalone
SIGMA/SRE0037 - aqueous solution, β-glucuronidase ≥100,000 units/mL
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
LC-MS analysis of codeine-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. The limpet-derived enzyme G8132 performed best with this substrate, with reaction completion within 3–4 hours at 60 °C at 1 unit per mL. Comparative data above was performed at 60 °C at 10 unit per mL.
LC-MS analysis of hydromorphone-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. Molluscan enzymes performed similarly. The reaction was complete within 3–4 hours at 60 °C at 1 unit per mL. The E. coli enzymes exhibited low specificity for this substrate.
| β-glucuronidase activity | ≥100,000 units/mL |
| form | aqueous solution |
| Quality Level | 200  |
| secondary activity | ≤8,000 units/mL sulfatase |
| storage temp. | 2-8°C |
| Application: | Beta-glucuronidase from abalone has been used as a standard to evaluate its performance in benzodiazepine liquid chromatography-mass spectrometry (LC-MS/MS)confirmation assays. |
| Application: | Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine. |
| Biochem/physiol Actions: | Beta-glucuronidase is anenzyme present in several animals, plants, and bacteria. It catalyzes thehydrolysis of Beta-glucuronide conjugates and plant glucuronides.Beta-glucuronidase is a member of the glycosyl hydrolases (GHs) family 2 and is ahomo-tetrameric glycoprotein. It facilitates the removal of glucuronic acidsfrom glucuronides and aids in the liver metabolism enzymes for the excretion ofendo- and xeno-biotics. |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay). |
| Packaging: | 25 mL in glass bottle |
| Physical form: | Supplied as an aqueous solution containing 0.85% NaCl and 0.02% sodium azide |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 23-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | β-glucuronidase activity: ≥100,000 units/mL; secondary activity: ≤8,000 units/mL sulfatase |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.2.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |