Catalase from bovine liver
SIGMA/SRE0041 - Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 2,000-5,000 units/mg protein
Synonym: Catalase from bovine liver; H2O2:H2O2 oxidoreductase
CAS Number: 9001-05-2
EC Number: 232-577-1
MDL Number: MFCD00081483
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: SRE0041-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: SRE0041-50G
| application(s) | diagnostic assay manufacturing |
| form | lyophilized powder |
| InChI | 1S/C9H10O3/c1-2-12-9(11)7 |
| InChI key | NUVBSKCKDOMJSU-UHFFFAOYSA |
| isoelectric point | 5.4 |
| mol wt | tetramer ~250 kDa |
| Quality Level | 400  |
| shipped in | wet ice |
| SMILES string | O(CC)C(=O)c1ccc(cc1)O |
| specific activity | 2,000-5,000 units/mg protein |
| storage temp. | −20°C |
| Application: | Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli. Catalase from bovine liver may be used:
|
| Biochem/physiol Actions: | Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT. |
| General description: | Research Area: Cell Signaling Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms. |
| Other Notes: | One unit will decompose 1.0 micromole of hydrogen peroxide per minute at pH 7.0 and 25 deg C, while the hydrogen peroxide concentration falls from 10.3 to 9.2 millimolar. |
| Packaging: | 10, 50 g in poly bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 2,000-5,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.11.1.6 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |