Proteinase K from Tritirachium album
SIGMA/SRE0047 - Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥30 units/mg protein, Molecular Biology
Synonym: Endopeptidase K
CAS Number: 39450-01-6
EC Number: 254-457-8
MDL Number: MFCD00132129
Product Type: Chemical
Proteinase K illustration.
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| application(s) | diagnostic assay manufacturing |
| biological source | fungus (Tritirachium album) |
| color | white |
| foreign activity | DNAse, Nickase and RNAse, none detected |
| form | lyophilized powder |
| grade | Molecular Biology |
| impurities | ≤1 ppm DNA |
| mol wt | 28,930 Da |
| 28.93 kDa | |
| pH | 7.5 |
| Quality Level | 400  |
| shipped in | wet ice |
| specific activity | ≥30 units/mg protein |
| storage condition | dry at room temperature |
| storage temp. | −20°C |
| technique(s) | DNA extraction: suitable |
| RNA extraction: suitable |
| Application: | Proteinase K catalyzes the hydrolysis of keratin. |
| Application: | Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. |
| Application: | Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. |
| Biochem/physiol Actions: | Proteinase K catalyzes the hydrolysis of keratin. |
| Biochem/physiol Actions: | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
| General description: | Proteinase K is an extracellular endopeptidase. It is synthesized by the mold Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein with a molecular weight of 28,930 Da. Active proteinase K is characterized with an unhydrolysed protein chain and autolysed polypeptide chains. |
| Other Notes: | One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 |
| Precautionary statements | P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| activity | specific activity: ≥30 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.64 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |