Lysostaphin from Staphylococcus staphylolyticus
SIGMA/SRE0053 - Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder
Synonym: Glycyl-glycine Endopeptidase
CAS Number: 9011-93-2
MDL Number: MFCD00131556
Product Type: Chemical
Lysostaphin has endopeptidase activity, which cleaves polyglycine cross-links in the cellular wall of Staphylococcus species. Under certain conditions preparations may also exhibit hexosaminidase activity (specific for the glucosaminyl-muramic acid bond of the bacterial carbohydrate backbone, but does not react with S. aureus) and amidase activity.
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial ( |
| composition | protein, 50-70% biuret |
| concentration | 50—70% protein |
| form | lyophilized powder |
| mol wt | 26,926 Da |
| packaging | pkg of (package size sold on basis of mg protein) |
| Quality Level | 400  |
| shipped in | wet ice |
| specific activity | ≥500 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for microbiological tests |
| Biochem/physiol Actions: | Lysostaphin has the ability to cleave both viable and heat-killed S. aureus cells. It is one of the major antibacterial agents for the staphylococci. Lysostaphin cleaves the cross-linking pentaglycine bridges in the cell walls of staphylococci. Thus it is considered as an antistaphylococcal therapeutic agent. |
| Biochem/physiol Actions: | Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5 |
| General description: | Lysostaphin is a glycylglycine endopeptidase. It is an important antibacterial enzyme. |
| Other Notes: | One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH7.5 at 37°C in a 6.0ml reaction mixture. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥500 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.75 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |