Tris-Borate EDTA Buffer, 10X Concentrate
SIGMA/SRE0062
Synonym: TBE Buffer
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| form | liquid |
| Quality Level | 500  |
| storage temp. | room temp |
| Application: | Tris-Borate EDTA (TBE) buffer is commonly used in the electrophoresis of nucleic acids in agarose and polyacrylamide gels. TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp. |
| Physical form: | 10X TBE buffer stock solution contains 0.89 M Trizma(R) with 0.02 M EDTA adjusted to pH 8.3 with boric acid. Dilute with ultrapure water to 1X. Notes: If precipitation is present in the 10X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution. It is recommended 1X working solutions be filtered through a 0.2 mm filter before use. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H360FD |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41105319 |