Peroxidase from horseradish
SIGMA/SRE0082 - For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US, Type VI, powder, stabilized, ≥250 units/mg solid (using pyrogallol)
Synonym: Peroxidase from horseradish; horse radish peroxidase; horseradish peroxidase; horseradish peroxidase (hrp); hrp; peroxidase; peroxidase enzyme; peroxidase from horseradish; Donor:hydrogen-
CAS Number: 9003-99-0
EC Number: 232-668-6
MDL Number: MFCD00071339
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: SRE0082-30KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: SRE0082-5KU
Elevate Quality Program Icon
| application(s) | diagnostic assay manufacturing |
| color | brown |
| form | powder, stabilized |
| InChI | 1S/H2O3/c1-3-2/h1-2H |
| InChI key | JSPLKZUTYZBBKA-UHFFFAOYSA |
| Quality Level | 400  |
| shipped in | wet ice |
| SMILES string | [O+H2]O[O-] |
| solubility | phosphate buffer: soluble |
| specific activity | ≥250 units/mg solid (using pyrogallol) |
| storage temp. | 2-8°C |
| suitability | suitable for immunoassay |
| suitable for manufacturing use | |
| technique(s) | ELISA: suitable |
| immunoassay: suitable | |
| lateral flow assay: suitable | |
| western blot: suitable | |
| type | Type VI |
| Analysis Note: | The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity. |
| Application: | HRP is used in immunoassays, such as ELISAs, Western blots, and lateral flow assays. HRP is used in academic research, diagnostics, and pharmaceutical research. For more applications, see the following technical article: An Introduction to Horseradish Peroxidase (HRP) and Its Applications . |
| Application: | Peroxidase from horseradish has been used in western blotting. |
| General description: | Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). HRP is a single chain polypeptide containing four disulfide bonds. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes is 3.0 - 9.0. |
| General description: | Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. |
| Other Notes: | One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C. |
| Other Notes: | View more information on peroxidase at www.sigma-aldrich.com/enz. |
| Packaging: | 5000, 30000, 300000 units in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥250 units/mg solid (using pyrogallol) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.11.1.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |