β-Glucuronidase from limpets (Patella vulgata)
SIGMA/SRE0093 - aqueous solution
Synonym: β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
Recombinantly expressed and chromatographically purified to eliminate MAM esterase activity and reduce potentially interfering substances.
Recombinant Limpet beta-glucuronidase (SRE0093 and SRE0095) exhibits superior thermal stability, retaining ~60% activity at 60 #°C for seven days.
Activity of recombinant limpet β-Glucuronidase (SRE0093 and SRE0095) was tested in reaction buffers of different pH values at 37 °C. Activity was measured as μg of phenolphthalein liberated per 10 minutes.
Activity of recombinant limpet β-Glucuronidase (SRE0093 and SRE0095) was tested at different reaction temperatures at pH 3.8. Activity was measured as μg of phenolphthalein liberated per 10 minutes.
Recombinant Limpetβ-glucuronidase (SRE0093 and SRE0095) exhibits exceptional stability in master mix, retaining >95% of activity after 43 days.
Hydrolysis of various drug conjugates using SRE0093 rLimpet β-glucuronidase at 50 U/mL, pH = 5.0, hydrolysis at 70 °C.
Hydrolysis using SRE0093 rLimpet β-glucuronidase at 50 U/mL, pH = 5.0, hydrolysis at 70 °C. Codeine-glucuronide spike level at 1,000 ng/mL in synthetic urine.
Hydrolysis using SRE0093 rLimpet β-glucuronidase at 50 U/mL, pH = 5.0, hydrolysis at 70 °C. Lorazapam-glucuronide spike level at 1,000 ng/mL in synthetic urine.
Hydrolysis using SRE0093 rLimpet β-glucuronidase at 50 U/mL, pH = 5.0, hydrolysis at 70 °C. Amitryptiline-glucuronide spike level at 1,000 ng/mL in synthetic urine.
Hydrolysis using SRE0093 rLimpet β-glucuronidase at 50 U/mL, pH = 5.0, hydrolysis at 70 °C. Carboxy-THC-glucuronide spike level at 1,000 ng/mL in synthetic urine.
Hydrolysis using SRE0093 rLimpet β-glucuronidase at 50 U/mL, pH = 5.0, hydrolysis at 70 °C. Testosterone-glucuronide spike level at 1,000 ng/mL in synthetic urine.
| concentration | 100000-200000 units/mL |
| form | aqueous solution |
| Quality Level | 300  |
| recombinant | expressed in proprietary host |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Application: | New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine |
| Application: | More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page . |
| Application: | Poster presentation comparing the hydrolysis efficiency of novel beta-glucuronidase enzymes from recombinant limpet and recombinant E. coli sources, click at the following poster link: Hydrolysis Efficiency Evaluation of Novel Recombinant Limpet and E. coli Beta-Glucuronidase Enzymes |
| Application: | The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes |
| Features and Benefits: | Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→mor |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay). |
| Physical form: | Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |