HDAC-1 human
SIGMA/SRP0100 - recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)
Synonym: HD1; Histone deacetylase 1
Product Type: Chemical
SDS-PAGE HDAC-1: Cat. No. SRP0100: Coomassie blue-stained SDS-PAGE analysis of HDAC2,FLAG tag
| assay | ≥50% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 56 kDa |
| NCBI accession no. | NM_004964   |
| packaging | pkg of 50 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q13547 |
| Biochem/physiol Actions: | Useful for the study of enzyme kinetics, screening inhibitors. |
| General description: | Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal HIS-DDDDK tag (FLAG) and C-terminal His-tag, MW = 56 kDa, expressed in baculovirus expression system. |
| Other Notes: | One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥50% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |