HDAC-3/NCOR2 human
SIGMA/SRP0104 - recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
Synonym: HD3; Histone deacetylase 3; RPD3; SMAP45
Product Type: Chemical
assay | ≥90% (SDS-PAGE) |
biological source | human |
concentration | >0.02 mg/mL |
form | aqueous solution |
mol wt | 37.6 kDa (NCOR2) |
49.7 kDa (HDAC3) | |
NCBI accession no. | NM_003883 ![]() ![]() |
packaging | pkg of 50 μg |
recombinant | expressed in baculovirus infected insect cells |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | O15379 ![]() ![]() |
Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
General description: | Complex of human HDAC3, GenBank Accession No. NM_003883, full length with C-terminal His tag, MW = 49.7 kDa. Human NCOR2, amino acid 395-489, GenBank Accession No. NM_006312, N-terminal ST tag, MW = 37.6 kDa, co-expressed in baculovirus expression system. |
Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 20 mM glutathione and 10% glycerol. |
Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
Unit Definition: | One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥90% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |