HDAC-7 human
SIGMA/SRP0109 - recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym: HD7; HD7A; HDAC7A; Histone deacetylase 7
Product Type: Chemical
SDS-PAGE HDAC-7: Cat. No. SRP0109: Coomassie blue-stained SDS-PAGE analysis of HDAC-7
Chart HDAC-7: Cat. No. SRP0109: Activity Assay of HDAC-7,
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 78 kDa |
| NCBI accession no. | AY302468   |
| packaging | pkg of 2 and 10 μg |
| potency | ≥10 units/mg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q8WUI4 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | Histone deacetylases (HDACs) play crucial roles in the epigenetic modulation of gene expression and helps in the growth, differentiation, and apoptosis of cancer cells. Knockdown of HDAC7 stimulates apoptosis and autophagy in salivary mucoepidermoid carcinoma cells. It may play a vital role in both types of B lymphopoiesis. HDAC7 serves as a repressor of myeloid genes. |
| General description: | HDAC7 (histone deacetylase 7) is located on human chromosome 12q13. It is expressed in fetal and adult pre-B cells. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C. |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |