HDAC-10 human
SIGMA/SRP0112 - recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)
Synonym: HD10; Histone deacetylase 10
Product Type: Chemical
SDS-PAGE HDAC-10: Cat. No. SRP0112: Coomassie blue-stained SDS-PAGE analysis of HDAC-10
Chart HDAC-10: Cat. No. SRP0112: Activity Assay of HDAC-10,
| assay | ≥10% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 79 kDa |
| NCBI accession no. | NM_032019   |
| packaging | pkg of 50 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q969S8 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human HDAC10, amino acids 1-481, GenBank Accession No. NM_032019, with N-terminal GST tag, MW = 79 kDa, expressed in baculovirus expression system. |
| Other Notes: | One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Purity | ≥10% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |