Sirtuin 1 human
SIGMA/SRP0115 - recombinant, expressed in E. coli, ≥60% (SDS-PAGE)
Synonym: SIR2; SIRT1; Sir2A; sir2-like 1
Product Type: Chemical
assay | ≥60% (SDS-PAGE) |
biological source | human |
form | aqueous solution |
mol wt | 87.2 kDa |
NCBI accession no. | NM_012238 |
packaging | pkg of 100 μg |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | Q96EB6 |
Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
General description: | Human Sirtuin 1, GenBank Accession No. NM_012238), amino acids 193-741 with N-terminal ST tag, MW = 87.2 kDa, expressed in E. coli expression system. |
Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol. |
Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
Unit Definition: | One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥60% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |