LSD1 Active human
SIGMA/SRP0122 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym: Amine oxidase (flavin containing) domain 2 (AOF2); BRAF35-HDAC complex protein BHC110; KIAA0601; Lysine (K)-specific demethylase 1 (LSD1); Lysine-specific histone demethylase 1A (KDM1A)
Product Type: Chemical
SDS-PAGE LSD1: Cat. No. SRP0122: Coomassie blue-stained SDS-PAGE analysis of LSD1
Chart LSD1: Cat. No. SRP0122: Activity Assay of LSD1
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 103 kDa |
| NCBI accession no. | NM_015013   |
| packaging | pkg of 50 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | O60341 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human LSD1, AOF2, amine oxidase, flavin containing, domain 2, GenBank Accession No. NM_015013, amino acids 158-end with N-terminal ST tag, MW = 103 kDa, expressed in E. coli expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C. |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |