LSD1 substrate (Di-methylated K4_H3)
SIGMA/SRP0125 - ≥90% (HPLC)
Synonym: HIST1H3E; Histone H3.1
Product Type: Chemical
| assay | ≥90% (HPLC) |
| biological source | human |
| concentration | 0.55 mg/mL |
| form | aqueous solution |
| mol wt | 2283 Da |
| packaging | pkg of 500UL |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P68431   |
| Application: | Study enzyme kinetics and screen small molecular inhibitors of LSD1 for drug discovery and HTS applications |
| Biochem/physiol Actions: | Histones have an important role in the organization and modification of chromatin. Histone cluster 1 H3 family member E (HIST1H3E) has a role in DNA synthesis during DNA replication and might also be involved in DNA repair. It is deposited at the chromatin at the time of DNA replication-associated chromatin assembly. During herpes simplex virus 1 (HSV-1) infection, differential movement of HIST1H3E is associated with the assembly of viral chromatin. |
| General description: | Lysine-specific demethylase 1 (LSD1) acts on mono- and dimethylated histones and demethylates them. It has a role in embryogenesis, cell proliferation, spermatogenesis, adipogenesis and chromosomal segregation. Histone H3 peptide methylated at Lysine 4 is a substrate for LSD1. Histone cluster 1 H3 family member E (HIST1H3E) is a variant of histone 3 and is also called as the replicative histone. It is mainly expressed in the S-phase of the cell cycle. The gene encoding this protein is localized on human chromosome 6p22. |
| Physical form: | Supplied as 500 uL of water solution at 200 uM concetration. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube to recover full content of the tube. Aliquot into single use aliquots. Store remaining product in aliquots at -70°C. Note: Avoid freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |