EZH1/EED/SUZ12/RbAp48/AEBP2 human
SIGMA/SRP0131 - recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
Synonym: ENX-2; EZH1; Enhancer of zeste homolog 1; KMT6B; Lysine N-methyltransferase 6B
Product Type: Chemical
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 48 kDa (RbAp48) |
| 51 kDa (EED) | |
| 53 kDa (AEBP2) | |
| 86 kDa (EZH1) | |
| 87 kDa (SUZ12) | |
| NCBI accession no. | NM_001991 (EZH1)   |
| NM_003797 (EED) |
|
| NM_005610 (RbAP48) |
|
| NM_015355 (SUZ12) |
|
| NM_153207 (AEBP2) |
|
| packaging | pkg of 50 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q92800 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Complex of: Human EZH1, GenBank Accession No. NM_001991, amino acids 2-end, with N-terminal His tag, MW = 86 kDa. Human EED, NM_003797, amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag. RbAp48 and AEBP2. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C. |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |