G9a human
SIGMA/SRP0136 - recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym: EHMT2; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; KMT1C; Lysine N-methyltransferase 1C
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
biological source | human |
concentration | >0.02 mg/mL |
form | aqueous solution |
mol wt | 74.6 kDa |
NCBI accession no. | NM_006709 |
packaging | pkg of 20 μg |
recombinant | expressed in baculovirus infected insect cells |
shipped in | dry ice |
storage temp. | −70°C |
UniProt accession no. | Q96KQ7 |
Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
General description: | Human G9a, euchromatic histone-lysine N-methyltransferase 2, GenBank Accession No. NM_006709, amino acids 785-1210, with N-terminal GST tag, MW = 74.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT. |
Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
Unit Definition: | One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C. |
Purity | ≥70% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |