PRMT1 Active human
SIGMA/SRP0141 - recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym: ANM1; Arginine methyltransferase 1; HMT1 hnRNP methyltransferase-
Product Type: Chemical
SDS-PAGE PRMT1 (expressed Sf9): Cat. No. SRP0141: Coomassie blue-stained SDS-PAGE analysis of PRMT1 (expressed Sf9)
Chart PRMT1 (expressed Sf9): Cat. No. SRP0141: Activity Assay of PRMT1
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 68 kDa |
| NCBI accession no. | NM_001536   |
| packaging | pkg of 20 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q99873 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human PRMT1, GenBank Accession No. NM_001536, amino acids 2-end, with N-terminal GST tag, MW = 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C. |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |