PRMT5/MEP50 Active human
SIGMA/SRP0146 - recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym: Arginine methyltransferase 5; HMT1 hnRNP methyltransferase-
Product Type: Chemical
SDS-PAGE PRMT5 (Sf9): Cat. No. SRP0146: Coomassie blue-stained SDS-PAGE analysis of PRMT5 (Sf9)
Chart PRMT5 (Sf9): Cat. No. SRP0146: Activity Assay of PRMT5 (Sf9)
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 73 kDa |
| NCBI accession no. | NM_006109   |
| NM_024102 |
|
| packaging | pkg of 20 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | O14744 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | Protein arginine methyltransferase 5 (PRMT5) catalyzes the symmetric dimethylation of arginine residues. It is implicated in various biological functions such as ribosome biogenesis, assembly of the Golgi apparatus, cellular differentiation, cellular proliferation, apoptosis, and germ cell specification. Irregular expression of PRMT5 leads to several type of cancers such as leukemia and lymphoma, gastric cancer, breast cancer and colorectal cancer. PRMT5 plays an important role in the regulation of cell growth, proliferation and apoptosis in epithelial ovarian cancer cell lines A2780 and SKOV3. PRMT5 enables nuclear factor-κB (NF-κB) activation via demethylating arginine 30 (R30) on the p65 subunit of NF-κB. PRMT5 participates in transcriptional regulation by methylating histones H2A, H3R8, and H4R3 as well as by inhibiting the expression of suppressor of tumorigenicity 7 (ST7), and nonmetastatic 23 (NM23) genes. PRMT5 enhances DNA damage response via methylation of tumor suppressor p53 on R333, R335, and R337 in the oligomerization domain. |
| General description: | Protein arginine methyltransferase 5 (PRMT5) also referred to as Janus kinase-binding protein 1, is encoded by the gene mapped to human chromosome 14q11.2. PRMT5 enzyme, containing 637 amino acids, belongs to type II enzymes, which is a subclass of PRMT family. PRMT5 is predominantly expressed at distinct level in various organs including heart, muscle and testis. Human PRMT5 (GenBank Accession No. NM_006109), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 73kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |