Set7/Set9 human
SIGMA/SRP0149 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym: KMT7; Lysine N-methyltransferase 7; SET domain containing (lysine methyltransferase) 7; SET7/9; SETD7
Product Type: Chemical
SDS-PAGE Set7/Set9: Cat. No. SRP0149: Coomassie blue-stained SDS-PAGE analysis of Set7/Set9
Chart Set7/Set9: Cat. No. SRP0149: Activity Assay of Set7/Set9
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 61 kDa |
| NCBI accession no. | NM_030648   |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q8WTS6 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | SET7/9 acts as a histone methyltransferase and catalyzes monomethylation of histone H3 at lysine 4 (H3K4me1) in vitro. Down-regulation of SET7/9 expression affects human embryonic stem cell differentiation. The encoded protein is implicated in the regulation of proteins and transcription factor activity. It also stimulates promoters of methylation-dependent co-recruitment through non-histone protein methylation. Mutation in the gene encoding SET7/9 is associated with serum lycopene concentrations. Elevated expression of the gene is associated with progression of hepatocellular carcinoma (HCC). |
| General description: | Human recombinant SET7/9, amino acids 52-end, with N-terminal GST tag, GenBank Accession No. NM_030648, expressed in a E. coli expression system. MW = 61 kDa, |
| General description: | SET7/9 also known as KMT7/SET domain containing lysine methyltransferase 7 (SETD7), is encoded by the gene mapped to human chromosome 4q31.1. The encoded protein belongs to SET domain-containing protein family. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |