JMJD2B Active human
SIGMA/SRP0169 - recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)
Synonym: JHDM3B; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B; KIAA0876; Lysine-specific demethylase 4B (KDM4B)
Product Type: Chemical
SDS-PAGE JMJD2B: Cat. No. SRP0169: Coomassie blue-stained SDS-PAGE analysis of JMJD2B
Chart JMJD2B: Cat. No. SRP0169: Activity Assay of JMJD2B
| assay | ≥40% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 57 kDa |
| NCBI accession no. | NM_015015   |
| packaging | pkg of 100 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | O94953 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system. |
| Other Notes: | One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Purity | ≥40% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |