Jak2 Jh1 Active human
SIGMA/SRP0171 - recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
Synonym: JAK2; JTK10; Janus kinase 2; Just another kinase 2; Tyrosine-protein kinase JAK2
Product Type: Chemical
SDS-PAGE Jak2 Jh1: Cat. No. SRP0171: Coomassie blue-stained SDS-PAGE analysis of Jak2 Jh1
Chart Jak2 Jh1: Cat. No. SRP0171: Activity Assay of Jak2 Jh1
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 42.5 kDa |
| NCBI accession no. | NM_004972   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | O60674 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human Jak2 (GenBank Accession No. NM_004972), (amino acids. 808-end) with N-terminal His tag, MW = 42.5 kDa, expressed in Baculovirus expression system. |
| Other Notes: | One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 1 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |