Jak3 Active human
SIGMA/SRP0173 - recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonym: JAK3; Janus kinase 3; Just another kinase 3; LJAK; Leukocyte Janus kinase
Product Type: Chemical
SDS-PAGE Jak3: Cat. No. SRP0173: Coomassie blue-stained SDS-PAGE analysis of Jak3
Chart Jak3: Cat. No. SRP0173: Activity Assay of Jak3
| assay | ≥60% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 65 kDa |
| NCBI accession no. | NM_000215   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P52333 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human Jak3 (GenBank Accession No. NM_000215), (amino acids 781-end) with N-terminal His tag, MW = 37 kDa, expressed in Baculovirus expression system. |
| Other Notes: | One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 1 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P201 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥60% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |