Histone H4 full length human
SIGMA/SRP0178 - recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym: HIST2H4A
Product Type: Chemical
SDS-PAGE Histone H4, full length: Cat. No. SRP0178: Coomassie blue-stained SDS-PAGE analysis of Histone H4, full length
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 12.1 kDa |
| NCBI accession no. | NM_003548   |
| packaging | pkg of 1 mg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P62805 |
| Application: | Suitable substrate for histone methyltransferases. |
| Biochem/physiol Actions: | Histones H2B and H4 are found to become mobilized during an infection with HSV-1 (herpes simplex virus-1) and become available for binding to viral genome. |
| General description: | Human Histone 4 (HIST2H4A) (GenBank Accession No. NM_003548), amino acids 2-104 (end) with N-terminal His-tag, MW = 12.1 kDa, expressed in an E. coli expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |