Lysyl Oxidase-like 2 (LOXL2) FLAG-tag (Sf9-derived) human
SIGMA/SRP0179 - recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)
Synonym: LOR2; LOXL2; WS9-14
Product Type: Chemical
SDS-PAGE Lysyl Oxidase-like 2 (LOXL2): Cat. No. SRP0179: Coomassie blue-stained SDS-PAGE analysis of Lysyl Oxidase-like 2 (LOXL2)
| assay | ≥65% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| NCBI accession no. | NM_002318   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q9Y4K0 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions: | LOXL2 (lysyl oxidase like 2) is an amino oxidase which is dependent on copper and quinone. LOXL2 participates in various events, such as gene transcription, cell migration, adhesion, angiogenesis and differentiation. It is responsible for connecting collagen and/or elastin in the extracellular matrix. It deaminates trimethylated lysine 4 in histone H3, causing transcriptional repression. LOXL2 also oxidises methylated TAF10 (TATA-box binding protein associated factor 10), suppressing some TFIID (transcription factor IID)-associated genes. The LOXL2 gene is upregulated in various cancers, such as gastric cancer, breast cancer and squamous cell carcinomas. The gene is associated with cancer cell invasion, lymph node metastasis and poor overall patient survival. |
| General description: | The gene LOXL2 (lysyl oxidase like 2) is mapped to human chromosome 8p21.3. It is widely expressed and belongs to the LOX family of proteins. The protein has a conserved C-terminus region with catalytic function and a divergent N-terminal part. Human LOXL2 (GenBank Accession No. NM_002318), amino acids 500-end, MW = 32kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥65% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |