PARP2 Active human
SIGMA/SRP0193 - recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonym: ADPRT2; ADPRTL3; NAD(+) ADP-
Product Type: Chemical
Chart PARP2: Cat. No. SRP0193: Activity Assay of PARP2
| application(s) | life science and biopharma |
| assay | ≥60% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| manufacturer/tradename | Sigma-Aldrich |
| mol wt | 92 kDa |
| NCBI accession no. | NM_005484   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| solubility | water: soluble |
| storage temp. | −70°C |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | Q9UGN5 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Other Notes: | One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥60% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |