PTP1B (1-321)/PTPN1 Active from rat
SIGMA/SRP0214 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: PTPN1; Protein tyrosine phosphatase, non-receptor type 1
Product Type: Chemical
| assay | ≥90% (SDS-PAGE) |
| biological source | rat |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 63.3 kDa |
| NCBI accession no. | NM_012637.2   |
| packaging | pkg of 20 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P20417 |
| Application: | Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. |
| General description: | Protein tyrosine phosphatase, catalytic domain (amino acids. 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system. |
| Physical form: | Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |