SHP-1 Active human
SIGMA/SRP0216 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: HCP; Hematopoietic cell phosphatase; PTP-1C; PTPN6; SHP-1L
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | 1 mg/mL |
| form | aqueous solution |
| mol wt | 95 kDa |
| NCBI accession no. | NM_080549   |
| packaging | pkg of 20 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P29350 |
| Application: | Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. |
| Biochem/physiol Actions: | In mammary epithelial cells, protein tyrosine phosphatase, non-receptor type 6 (PTPN6) controls EGFR (epidermal growth factor receptor) signaling. It participates in the JAK/STAT (janus kinase/signal transducers and activators of transcription) signaling pathway. PTPN6 regulates cytokine receptors like interleukin- 3 (IL-3) and erythropoietin. |
| General description: | Protein tyrosine phosphatase, full length, (Gene bank accession number NM_080549) with N-terminal GST tag, MW = 95 kDa, expressed in an E. coli expression system. |
| General description: | The Src homology region 2 domain-containing phosphatase-1 (SHP1)/protein tyrosine phosphatase, non-receptor type 6 (PTPN6) is a 68 kDa protein present in the cytoplasm. It is mapped to human chromosome 12p13. |
| Other Notes: | One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |