SHP-2 Active human
SIGMA/SRP0217 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: Noonan syndrome 1; PTP-1D; PTP2C; PTPN11; SHP2
Product Type: Chemical
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | 0.73 mg/mL |
| form | aqueous solution |
| mol wt | 59.5 kDa |
| NCBI accession no. | NM_002834   |
| packaging | pkg of 20 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q06124 |
| Application: | Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme. |
| Biochem/physiol Actions: | Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) or SHP-2 is involved in signal transduction pathways downstream of growth receptors. It acts as a proto-oncogene. SHP-2 leads to sustained RAS activation by dephosphorylating the RAS-GTPase-activating protein binding site on Grb2-associated binder 1 (Gab1). This protein also nucleates multiple signaling proteins and thus, acts as an adaptor protein. Mutations in the SHP-2 gene have been associated with Noonan syndrome. |
| General description: | Protein tyrosine phosphatase, non-receptor type 11 (PTPN11), also known as SHP-2, is a scaffolding protein. It possesses two SH2 domains at the amino terminal, a protein tyrosine phosphatase domain and carboxy-terminal region. The gene encoding SHP-2 is localized on human chromosome 12q24.13. |
| Other Notes: | Amino acids : 224-529 |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |