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SHP-2 Active human

SIGMA/SRP0217 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: Noonan syndrome 1; PTP-1D; PTP2C; PTPN11; SHP2

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SRP0217-20UG 20 µg
$480.00
1/EA
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45-SRP0217-50UG
No Price  
assay ≥90% (SDS-PAGE)
biological source human
concentration 0.73 mg/mL
form aqueous solution
mol wt 59.5 kDa
NCBI accession no. NM_002834 
packaging pkg of 20 μg
recombinant expressed in E. coli
shipped in dry ice
storage condition avoid repeated freeze/thaw cycles
storage temp. −70°C
UniProt accession no. Q06124 
Application: Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme.
Biochem/physiol Actions: Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) or SHP-2 is involved in signal transduction pathways downstream of growth receptors. It acts as a proto-oncogene. SHP-2 leads to sustained RAS activation by dephosphorylating the RAS-GTPase-activating protein binding site on Grb2-associated binder 1 (Gab1). This protein also nucleates multiple signaling proteins and thus, acts as an adaptor protein. Mutations in the SHP-2 gene have been associated with Noonan syndrome.
General description: Protein tyrosine phosphatase, non-receptor type 11 (PTPN11), also known as SHP-2, is a scaffolding protein. It possesses two SH2 domains at the amino terminal, a protein tyrosine phosphatase domain and carboxy-terminal region. The gene encoding SHP-2 is localized on human chromosome 12q24.13.
Other Notes: Amino acids : 224-529
Physical form: Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
Preparation Note: Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥90% (SDS-PAGE)
Storage Temp. −70°C
UNSPSC 12352200

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