TC-PTP Active human
SIGMA/SRP0218 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: PTPN2; T-cell protein-tyrosine phosphatase; TC-PTP
Product Type: Chemical
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | 2.9 mg/mL |
| form | aqueous solution |
| mol wt | 62.3 kDa |
| NCBI accession no. | BC016727   |
| packaging | pkg of 20 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P17706 |
| Application: | Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. |
| General description: | Protein tyrosine phosphatase, Catalytic domain, (amino acids 2-315), (Gene bank accession number BC016727) with N-terminal GST tag, MW = 62.3 kDa, expressed in an E. coli expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |