DEP1 Active human
SIGMA/SRP0220 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: CD148; DEP-1; Density-enhanced phosphatase 1; PTPRJ; Protein tyrosine phosphatase, receptor type, J
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 58.9 kDa |
| NCBI accession no. | NM_U10886   |
| packaging | pkg of 20 μg |
| potency | ≥0.114 units/mg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q12913 |
| Application: | Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates. |
| General description: | Protein tyrosine phosphatase (density-enhanced phosphatase-1, PTP-eta, CD148), Catalytic domain, (amino acids 1024-1306) (Gene bank accession no. NM_U10886) with N-terminal GST tag, MW=58.9 kDa, expressed in an E. coli expression system. |
| Other Notes: | One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |