SMURF1 Active human
SIGMA/SRP0227 - recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
Synonym: E3 ubiquitin ligase SMURF1; KIAA1625; SMAD specific E3 ubiquitin protein ligase 1
Product Type: Chemical
assay | ≥80% (SDS-PAGE) |
biological source | human |
concentration | >0.02 mg/mL |
form | aqueous solution |
mol wt | 70 kDa |
NCBI accession no. | NM_020429 |
packaging | pkg of 20 μg |
recombinant | expressed in baculovirus infected insect cells |
shipped in | dry ice |
specific activity | ≥0.0055 pmol/min-μg |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −70°C |
UniProt accession no. | Q9HCE7 |
Application: | Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
General description: | Human SMURF 1 (GenBank Accession No. NM_020429), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 70 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT. |
Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
Unit Definition: | One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C. |
Purity | ≥80% (SDS-PAGE) |
activity | specific activity: ≥0.0055 pmol/min-μg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |