WWP1 Active human
SIGMA/SRP0229 - recombinant, expressed in baculovirus infected insect cells, ≥15% (SDS-PAGE)
Synonym: AIP5; Atrophin-1 interacting protein 5; NEDD4-like E3 ubiquitin-protein ligase; TGIF-interacting ubiquitin ligase 1 (Tiul1); WW domain containing E3 ubiquitin protein ligase 1
Product Type: Chemical
SDS-PAGE WWP1: Cat. No. SRP0229: Coomassie blue-stained SDS-PAGE analysis of WWP1
| assay | ≥15% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 106 kDa |
| NCBI accession no. | NM_007013   |
| packaging | pkg of 20 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q9H0M0 |
| Application: | Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human WWP1 (GenBank Accession No. NM_007013), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 106 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Other Notes: | One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Purity | ≥15% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |