PI3 kinase (p110a/p85a) Active human
SIGMA/SRP0233 - recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
Synonym: PIK3CA; Phosphoinositide-
Product Type: Chemical
assay | ≥80% (SDS-PAGE) |
biological source | human |
concentration | >0.02 mg/mL |
form | aqueous solution |
mol wt | 129 kDa (p110α) |
88 kDa (p85α) | |
NCBI accession no. | U79143 (p110alpha) ![]() ![]() |
XM_043865 (p85alpha) ![]() ![]() |
|
packaging | pkg of 20 μg |
recombinant | expressed in baculovirus infected insect cells |
shipped in | dry ice |
specific activity | ≥13 pmol/min-μg |
storage temp. | −70°C |
UniProt accession no. | P42336 ![]() ![]() |
Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Biochem/physiol Actions: | PIK3CA (phosphatidylinositol-4,5 |
General description: | PIK3CA (phosphatidylinositol-4,5 |
Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT. |
Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
Unit Definition: | One unit of 110a/p85a kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C. |
Purity | ≥80% (SDS-PAGE) |
activity | specific activity: ≥13 pmol/min-μg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |