PI3 kinase (p110a/p85a) Active human

SIGMA/SRP0233 - recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: PI3-kinase regulatory subunit 4; PIK3R4; Phosphoinositide 3-kinase adaptor protein; VPS15; PIK3CA; Phosphoinositide-3-kinase, catalytic, alpha; PtdIns-3-kinase p110

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-SRP0233-20UG	20 µg	$761.00 1/EA	Add To Favorites

SDS-PAGE PI3 kinase (p110a/p85a): Cat. No. SRP0233: Coomassie blue-stained SDS-PAGE analysis of PI3 kinase (p110a/p85a)

Chart PI3 kinase (p110a/p85a): Cat. No. SRP0233: Activity Assay of PI3 kinase (p110a/p85a)

Properties
Descriptions
Basic Data

application(s)	life science and biopharma
assay	≥80% (SDS-PAGE)
biological source	human
concentration	>0.02 mg/mL
form	aqueous solution
manufacturer/tradename	Sigma-Aldrich
mol wt	129 kDa (p110α)
	88 kDa (p85α)
NCBI accession no.	U79143 (p110alpha)
	XM_043865 (p85alpha)
packaging	pkg of 20 μg
recombinant	expressed in baculovirus infected insect cells
shipped in	dry ice
specific activity	≥13 pmol/min-μg
storage temp.	−70°C
technique(s)	activity assay: suitable
UniProt accession no.	P42336

Application:	PI3 kinase (p110a/p85a) Active human has been used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions:	PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four catalytic subunits of PI3K protein, which plays an essential role in extracellular growth signaling. It phosphorylates and activates protein kinase B (PKB) protein. This protein has a wide range of physiological functions, such as proliferation, cell cycle, survival, adhesion, motility, apoptosis, angiogenesis, DNA repair, senescence, and cellular metabolism, and plays a central role in multiple types of cancers. It also functions as an intermediary in cellular signaling and is primarily recognized for its involvement in the Phosphoinositide 3-kinase/ Protein kinase B/ mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway. PIK3CA gene is either mutated or amplified in brain, breast, stomach, liver, ovary, and colon cancers. Mutation in this gene leads to enhanced PI3K activity, and it is a candidate transforming oncogene. It is over-expressed in invasive breast cancer and is a marker for poor prognosis. This gene is amplified in high-grade dysplastic lesions from bronchial biopsy specimens obtained from preinvasive squamous lung cancer. Frequency of mutations in the PIK3CA gene is higher in tumors without lymph node metastasis than in those with gastric cancer. Thus, it might be essential to evaluate the mutations in this gene to determine cetuximab treatment in gastric cancer patients.
General description:	Research area: Cell Signaling PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four subunits of the 110kDa catalytic subunit of PI3K protein, which contains a regulatory region of 85kDa. PI3K proteins form a family of lipid kinases. This gene is localized to human chromosome 3q.Three different classes of PI3Ks (I – III) have been discovered.
Other Notes:	One unit of 110a/p85a kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.
Physical form:	Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note:	Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Purity	≥80% (SDS-PAGE)
activity	specific activity: ≥13 pmol/min-μg
Storage Temp.	−70°C
UNSPSC	12352200

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