PI3 kinase [p110a(E545K)/p85a] Active human
SIGMA/SRP0234 - recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE)
Synonym: PIK3CA; Phosphoinositide-
Product Type: Chemical
Chart PI3 kinase [p110a(E545K)/p85a]: Cat. No. SRP0234: Activity Assay of PI3 kinase [p110a(E545K)/p85a]
| assay | ≥55% (SDS-PAGE) |
| biological source | human |
| concentration | 0.56 mg/mL |
| form | aqueous solution |
| mol wt | 129 kDa |
| 88 kDa | |
| NCBI accession no. | U79143 (p110alpha)   |
| XM_043865 (p85alpha) |
|
| packaging | pkg of 20 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| specific activity | ≥800 pmol/min-μg |
| storage temp. | −70°C |
| UniProt accession no. | P42336 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C. |
| Purity | ≥55% (SDS-PAGE) |
| activity | specific activity: ≥800 pmol/min-μg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |