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PI3 kinase [p110a(E545K)/p85a] Active human

SIGMA/SRP0234 - recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE)

Synonym: PIK3CA; Phosphoinositide-3-kinase, catalytic, alpha; PtdIns-3-kinase p110

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SRP0234-20UG 20 µg
$687.00
1/EA
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Chart PI3 kinase [p110a(E545K)/p85a]: Cat. No. SRP0234: Activity Assay of PI3 kinase [p110a(E545K)/p85a]

 

assay ≥55% (SDS-PAGE)
biological source human
concentration 0.56 mg/mL
form aqueous solution
mol wt 129 kDa
  88 kDa
NCBI accession no. U79143 (p110alpha) 
  XM_043865 (p85alpha) 
packaging pkg of 20 μg
recombinant expressed in baculovirus infected insect cells
shipped in dry ice
specific activity ≥800 pmol/min-μg
storage temp. −70°C
UniProt accession no. P42336 
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description: Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.
Physical form: Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note: Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Unit Definition: One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.
Purity ≥55% (SDS-PAGE)
activity specific activity: ≥800 pmol/min-μg
Storage Temp. −70°C
UNSPSC 12352200

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