cMet Active human
SIGMA/SRP0238 - recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonym: HGFR; Hepatocyte growth factor receptor; RCCP2; Scatter factor receptor; met Proto-oncogene
Product Type: Chemical
SDS-PAGE cMet: Cat. No. SRP0238: Coomassie blue-stained SDS-PAGE analysis of cMet
Chart cMet: Cat. No. SRP0238: Activity Assay of cMet
| assay | ≥60% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 74 kDa |
| NCBI accession no. | X54559   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P08581 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. |
| General description: | Human cMet (GenBank Accession No. X54559), (amino acids 956-1390) with N-terminal GST tag, MW = 74 kDa, expressed in an Baculovirus infected Sf9 cell expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 3 mM DTT and 20% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. |
| Purity | ≥60% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |