aRAF Active human
SIGMA/SRP0243 - recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
Synonym: A-Raf proto-oncogene; PKS2; v-Raf murine sarcoma 3611 viral oncogene homolog
Product Type: Chemical
SDS-PAGE aRAF: Cat. No. SRP0243: Coomassie blue-stained SDS-PAGE analysis of aRAF
| assay | ≥80% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 63.6 kDa |
| NCBI accession no. | NM_001654   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | P10398 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Purity | ≥80% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |