PLK2 Active human
SIGMA/SRP0249 - recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonym: Polo-like kinase 2; SNK; Serum-inducible kinase
Product Type: Chemical
SDS-PAGE PLK2: Cat. No. SRP0249: Coomassie blue-stained SDS-PAGE analysis of PLK2
Chart PLK2: Cat. No. SRP0249: Activity Assay of PLK2
| assay | ≥60% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | calculated mol wt 107 kDa |
| NCBI accession no. | NM_006622   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| specific activity | ≥3.6 pmol/min-μg |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q9NYY3 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST tag, expressed in a baculovirus infected Sf9 cell expression system. The theoretical MW is 107 kDa. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥60% (SDS-PAGE) |
| activity | specific activity: ≥3.6 pmol/min-μg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |