Cofilin 1 Active human
SIGMA/SRP0254 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: CFL1; Cofilin, non-muscle isoform; p18
Product Type: Chemical
SDS-PAGE Cofilin 1 : Cat. No. SRP0254: Coomassie blue-stained SDS-PAGE analysis of Cofilin 1
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 44 kDa |
| NCBI accession no. | NM_005507   |
| packaging | pkg of 200 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P23528 |
| Application: | Kinase substrate for the kinase assay; western blot |
| Biochem/physiol Actions: | Cofilin-l (CFL1) plays a vital role in the regulation of actin cytoskeleton reorganization. It is also implicated in cancer cell migration and invasion in several types of tumor tissues. Elevated expression of the gene is associated with the development of esophageal squamous cell carcinoma (ESCC). In addition, abnormal expression of the gene leads to vulvar cancer and thus, it can be considered as a potential therapeutic target for the same. CFL1 facilitates cytokinesis, cell motility and morphogenesis. Activated CFL1 helps in preventing cytoskeletal-associated defects in axonal development stimulated by Syns. |
| General description: | Cofilin 1 is ubiquitously expressed and has a molecular mass of ~19kDa. |
| General description: | Human recombinant Cofilin1 (2-end) with N-terminal GST tag (GenBank Accession No. NM_005507), expressed in a E. coli expression system. Cofilin 1, MW = 44 kDa, |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |