PDE1B Active human
SIGMA/SRP0258 - recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym: Calcium/
Product Type: Chemical
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| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 87 kDa |
| NCBI accession no. | NM_000924   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| specific activity | ≥2000 units/μg |
| storage temp. | −70°C |
| UniProt accession no. | Q01064 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Other Notes: | One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: ≥2000 units/μg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |