PDE1C Active from mouse
SIGMA/SRP0259 - recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym: Calcium/
Product Type: Chemical
| assay | ≥70% (SDS-PAGE) |
| biological source | mouse |
| concentration | >0.02 mg/mL |
| form | aqueous solution |
| mol wt | 100 kDa |
| NCBI accession no. | NM_011054   |
| packaging | pkg of 10 μg |
| recombinant | expressed in baculovirus infected insect cells |
| shipped in | dry ice |
| specific activity | ≥5000 units/μg |
| storage temp. | −70°C |
| UniProt accession no. | Q64338 |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| General description: | Mouse PDE1C (GenBank Accession No. NM_011054), full length with N-terminal GST tag, MW = 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Physical form: | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Unit Definition: | One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: ≥5000 units/μg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |