Cathepsin F Active human
SIGMA/SRP0290 - recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)
Synonym: CATSF; CATSF; CLN13; CLN13; CATSF
Product Type: Chemical
Chart Cathepsin F: Cat. No. SRP0290: Activity Assay of Cathepsin F
| assay | ≥90% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 53 kDa |
| NCBI accession no. | NM_003793   |
| recombinant | expressed in FreeStyle™ 293-F cells |
| shipped in | dry ice |
| solubility | soluble |
| water: soluble | |
| specific activity | ≥18 pmol/min-μg |
| storage temp. | −70°C |
| technique(s) | activity assay: suitable |
| Application: | Active human cathepsin F is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin F has been used in a study to assess its implications for the development of novel immunomodulators. Active human cathepsin F has also been used in a study to investigate microarray expression analysis and identification of serum biomarkers for Niemann-Pick disease. |
| Biochem/physiol Actions: | Cathepsin F (CtsF) are endopeptidases that play a role in lysosomal protein degradation. Beyond its involvement in protein recycling, CtsF has also been associated with immune responses mediated by Toll-like receptors (TLRs) and antigen processing. Additionally, mutation in the cathepsin F gene is related to the development of familial papillary thyroid cancer , type B Kufs disease, defective lysosomal degradation and abnormal autophagy, and Alzheimer′s disease. |
| General description: | Research area: CELL SIGNALING Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(amino acids 1-16) + cathepsin S (amino acids 20-end), with C-terminal HIS tag, MW = 53 kDa (more than 58 kDa after post-modification), expressed in FreeStyle 293-F cells. |
| Legal Information: | FreeStyle is a trademark of Invitrogen Corp. |
| Other Notes: | One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C |
| Physical form: | Formulated in 45 mM sodium acetate, 135 mM NaCl and 10% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥18 pmol/min-μg |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.4.22.41 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |