Cathepsin L Active human
SIGMA/SRP0291 - recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)
Synonym: CATL; Major excreted protein (MEP)
Product Type: Chemical
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| application(s) | life science and biopharma |
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 36 kDa |
| NCBI accession no. | NM_001912   |
| recombinant | expressed in FreeStyle™ 293-F cells |
| shipped in | dry ice |
| specific activity | ≥3900 pmol/min-μg |
| storage temp. | −70°C |
| suitability | suitable for molecular biology |
| technique(s) | inhibition assay: suitable |
| Application: | Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used:
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| Biochem/physiol Actions: | Cathepsin L is a cysteine protease, involved primarily in protein breakdown in the lysosome. Cathepsin L degrades nuclear transcription factors and may affect cell cycle regulation. Cathepsin L is also involved in the immune system, specifically in degrading the invariant chain during major histocompatibility complex (MHC) class II processing, a crucial step in antigen presentation. Its expression in the thymus has been demonstrated to be vital for the development of natural killer cells. Additionally, Cathepsin L contributes to recycling processes during axon outgrowth and synapse formation in the developing postnatal central nervous system. Proteases are associated with the development and progression of cancer. Therefore, cathepsin L may be a potential therapeutic target in cancer treatment. |
| General description: | Research area: Cell Signaling Cathepsin L is a papain-like cysteine protease and belongs to the Clan A, Family C1. It is composed of L domain of α-helix and an R domain of β-sheet in the spatial structure. Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells. |
| Legal Information: | FreeStyle is a trademark of Invitrogen Corp. |
| Other Notes: | One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C |
| Physical form: | Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥3900 pmol/min-μg |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |