Cathepsin S Active human
SIGMA/SRP0292 - recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)
Synonym: CTSS; MGC3886
Product Type: Chemical
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| application(s) | life science and biopharma |
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| mol wt | 37 kDa |
| NCBI accession no. | NM_004079.3   |
| recombinant | expressed in FreeStyle™ 293-F cells |
| shipped in | dry ice |
| specific activity | ≥8100 pmol/min-μg |
| storage temp. | −70°C |
| suitability | suitable for molecular biology |
| technique(s) | activity assay: suitable |
| Application: | Active human cathepsin S has been used: • to assess the pathogenesis of Alzheimer′s disease. • to investigate the optimization of selectivity of Azepanone-based inhibitors. • for the immunocytochemical detection of cathepsin-S in mouse samples. • for incubating mouse brain sections to test the impact of cathepsin-S on perineuronal nets (PNNs) integrity. |
| Biochem/physiol Actions: | Cathepsin S, as a lysosomal protease, facilitates the breakdown of unwanted and damaged proteins in the endo-lysosomal pathway. Moreover, it plays specific roles, such as contributing to major histocompatibility complex (MHC) class II antigen presentation by aiding in the degradation of the invariant chain. Dysregulation of cathepsin S has been associated with various pathological conditions, such as cancer, arthritis, and cardiovascular disease. Cathepsin S is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. It is also used as a biomarker for atherosclerosis, diabetes, adiposity, and atherogenesis. Moreover, it acts as a promising target against brain-penetrating and for the treatment of multiple sclerosis and neuropathic pain. |
| General description: | Research area: Cell Signaling Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide (amino acids 1-16) + cathepsin S (amino acids 17-331), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells. Cathepsin S belongs to the cysteine cathepsin protease family. It has limited tissue expression, being associated with antigen-presenting cells localized in lymph and spleen, as well as other immune cells like macrophages. Human cathepsin S is produced from its corresponding CTSS gene on chromosome 1q21 and is synthesized as a pre-proenzyme. |
| Legal Information: | FreeStyle is a trademark of Invitrogen Corp. |
| Other Notes: | One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C |
| Physical form: | Formulated in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 3 mM DTT, and 10% glycerol. |
| Preparation Note: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P201 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥8100 pmol/min-μg |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |