TGF-β1 Latent human
SIGMA/SRP0300 - recombinant, expressed in FreeStyle™ 293-F cells, ≥98% (SDS-PAGE)
Synonym: LAP; TGFb1; TGFbeta1; latency-associated peptide; transforming growth factor beta-1
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
SDS-PAGE TGF-β1 Latent Human: Cat. No. SRP0300: Coomassie blue-stained SDS-PAGE analysis of TGF-β1. Lanes 1. 5 μg TGF-β1. 2. Protein Marker.
Chart TGF-β1 Latent Human: Cat. No. SRP0300: Activity assay of TGF-β1 Latent Human.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| manufacturer/tradename | Invitrogen |
| mol wt | 25 kDa |
| NCBI accession no. | NM_000660   |
| packaging | pkg of 5 μg |
| recombinant | expressed in FreeStyle™ 293-F cells |
| shipped in | dry ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −70°C |
| UniProt accession no. | Q14766 |
| Application: | Useful for cell culture and for the study of signaling and apoptotic pathways. |
| Biochem/physiol Actions: | LTBP1 (latent transforming growth factor β binding protein 1) is an extracellular matrix protein which also controls TGF-β (transforming growth factor β) bioavailability. LTBP1 is mainly responsible for the folding and secretion of TGF-β, thereby adding it to the extracellular matrix. It is a target of HTRA1 (high temperature requirement protein A1), a serine protease involved in cellular signaling. |
| General description: | Human TGFβ1 (GenBank Accession No. NM_000660), amino acids 30-390 (end) with Cys33Ser mutation and N-terminal His-tag, disulfide-linked homodimers of TGFβ1 LAP and mature TGFβ1, MW= ~75kDa and 25kDa, under non-reducing conditions. LTBP1 (latent transforming growth factor β binding protein 1) belongs to the LTBP family of proteins. The protein has 18 epidermal growth factor (EGF)-like repeats and four LTBP-like repeats. The gene is mapped to human chromosome 2p22.3. |
| Legal Information: | FreeStyle is a trademark of Invitrogen Corp. |
| Physical form: | Lyophilized from a 0.2 μM filtered, aqueous solution. |
| Preparation Note: | Reconstitute in sterile PBS with 0.1% BSA. Stability: >6 months at –70°C (as a solid). Store reconstituted protein in aliquots at –80°C for up to 3 months. Avoid freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |